P-214: The Rate of In Vitro Oocyte Maturation,Fertilization and Embryo Development in Presence of Melatonin in Mice

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cumulus–oocyte complexes (COCs, group i) and denuded COC (d-COC, group ii). The oocytes were cultured in maturation medium with different dosages of melatonin (1 – 110 – 105 nM). The cumulus expansion and nuclear status were evaluated after 24 hours of in vitro maturation. The oocytes were used for in vitro fertilization (IVF). The fertilized oocytes were cultured in medium supplemented with different doses of melatonin. Results: The expansion (86.79%) and maturation (80.55%) rate of COCs increased in supplemented medium with 10 nM of melatonin Vs control group (73.33%, p<0.01), but oocytes without cumulus cells indicated higher maturation rate at higher melatonin doses (10 and 100 μM, 84.34 and 79.5% vs. 69.33% in control group (p<0.01). Fertlization rate was higher in treated medium with 1 μM of melatonin (93.75 %, p<0.01). The rate of cleavage and blastocyst formation was promoted in medium supplemented with 10 and 100 nM of melatonin (92.37 and 89.36% vs. 81.25% in control group (p<0.01). We observed a dose dependent response to melatonin treatment in this experiment. Conclusion: Exogenous melatonin can promote cumulus cell expansion, IVM, and embryo development. However we investigated a dose-dependent response in different stages of maturation and development. It may reflect sensitive rate of oocytes and embryos to culture conditions.